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Title   종양세포의 방사선 감수성을 예측하는 인자로서 방사선 조사 전후의 Telomerase 활성도 변화의 유용성 ( Usefulness of Change of Telomerase Activity as a Predictive Assay for Radiation Response )
Publicationinfo   2000 Jan; 032(06): 1109-1115.
Key_word   Radiation , Predictive assay , Telomerase activity
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Abstract   Purpose: A sensitive predictive assay is necessary to determine the total radiation dose according to sensitivity of individual cancer cell lines. This study is performed to determine whether the radiation sensitivity is correlated with the changes in telomerase activity after irradiation. Materials and Methods: Two colorectal cancer cell lines with different radiation sensitivity were used. In order to confirm the difference in radiation sensitivity, we used a calorimetric assay. Telomerase activities were measured using the PCR-based telomeric repeat amplification protocol (TRAP). Results: We confirmed the difference in radiation sensitivity between NCI-H630 and NCI-H716. Survival fractions at 2 Gy were 0.836 for NCI-H630 and 0.317 for NCI-H716. Telomerase activity increased after irradiation with NCI-H630, which was more resistant to radiation, whereas telomerase activity decreased with NCI-H730. But dose-dependent change of telomerase activity was not confirmed. Conclusion: Our results suggested that telomerase activity change after irradiation could be used as a predictive assay for radiation response. Further studies with different cell lines and tumor tissues are necessary.
저 자   우홍균(Hong Gyun Wu),김영주(Young Jue Kim),김일한(Il Han Kim),박찬일(Chaun Il Pauk),하성환(Sung Whan Ha)